Fig. 6: Genetic knockout or chemical inhibition of Uhrf1 attenuates Myc-driven HCC development in mice.
A Image of mouse liver tissues from the indicated mice 10 weeks after birth. B RT-qPCR analysis of Uhrf1 expression in livers isolated from mice as indicated. WT: wild type, NT: nontumor, T: tumor. C Representative H&E staining and UHRF1 staining of liver tissues from the indicated mice. D Body weight, liver weight, liver/body weight ratio, and number of nodules from the indicated mice were measured at 10 weeks of birth (n = 6 per group). E WB analysis of UHRF1, GLI1, CD44, and CD133 expression in CRL-8024 control cells and hinokitiol-treated cells (10–100 μM). F Study design (left). Image of mouse liver tissues from MycHKI/+ mice treated with vehicle (n = 6) or hinokitiol (n = 8) at 9 weeks of birth (right). G Liver weight and number of nodules from the indicated mice were measured at 9 weeks of birth. H Detection of UHRF1, GLI1, CD44, and CD133 expression in mouse liver tissues as indicated by WB analysis. I Relative mRNA expression of differentiation-associated genes in mouse liver tissues as indicated (n = 3). Mean ± SD. P values were determined using unpaired Student’s t test. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. Ns not significant.
