Fig. 4: Efferocytosis of end stage neutrophils disrupts osteoblastic differentiation. | Cell Death & Disease

Fig. 4: Efferocytosis of end stage neutrophils disrupts osteoblastic differentiation.

From: Efferocytosis by bone marrow mesenchymal stromal cells disrupts osteoblastic differentiation via mitochondrial remodeling

Fig. 4

A Experimental Schematic (B–D) Bone marrow-derived ST2 cells incubated with increasing concentrations of end stage neutrophils (PMN) for 24 h, then differentiated into osteoblasts using supplemented media for 21 days and stained for alkaline phosphatase (red) and Von Kossa. Representative Images of 3, quantified showing Mean+SD. *p < 0.05, **p < 0.01, (ANOVA). E Experimental Schematic for sorted samples (F, G) ST2 cells challenged with excess neutrophils (1:10) and then sorted alongside controls into PMN +/−, differentiated into osteoblasts using supplemented media for 21 days, and then stained for alkaline phosphatase and Von Kossa. Representative Images of 3, quantified showing Mean + SD. **p < 0.01, ****p < 0.0001, (ANOVA with post Tukey test). H Heat map of genes related to osteogenesis at 3 h or 24 h vs the control.

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