Fig. 3: METTL3-mediated m6A modification enhanced LINC00839 expression. | Cell Death & Disease

Fig. 3: METTL3-mediated m6A modification enhanced LINC00839 expression.

From: METTL3-mediated m6A modification of LINC00839 maintains glioma stem cells and radiation resistance by activating Wnt/β-catenin signaling

Fig. 3

A Hierarchical clustering of differentially “writers” expression in HNP1 and MES28 cells. B METTL3 expression in NSCs and GSCs was detected by western blot. C METTL3 expression in primary (P) and recurrent (R) GBM samples was detected by western blot. D METTL3 expression in primary (Pri.) and recurrent (Rec.) GBM samples was detected by qPCR. E GSCs were treated with control or METTL3 shRNA. LINC00839 expression was detected by qRT-PCR. **p < 0.001. F Total RNA m6A contents in LINC00839-knockdown GSCs was quantified by dot blot assays. G m6A enrichment in LINC00839 transcripts in control and METTL3-silencing cells using MeRIP-qPCR. H Primer-walking strategy. Five paired primers were designed according to the gene/exon–intron length. I MeRIP-qPCR assays using walking primers were performed to analyze the m6A-modification region. J MeRIP-qPCR assays to analyze the m6A-modification levels of LINC00839 in MES28 transfected with LINC00839 wildtype and its mutants expression. K MeRIP-qPCR assays to analyze the m6A-modification levels of LINC00839 in control or METTL3-depleted MES28 transfected with LINC00839 wildtype and its mutants expression.

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