Fig. 5: LINC00839 interacts with β-catenin in GSCs.

A Coomassie blue staining of the LINC00839–protein complex by incubation of biotinylated-LINC00839 with protein extracts from MES28. B The enrichment of β-catenin in pull-down products of LINC00839 was measured by western blot. C RNA immunoprecipitation with an anti-β-catenin antibody was used to assess whether β-catenin binding to LINC00839 in GSCs. D Molecular docking diagram of LINC00839 and β-catenin. Green: 445–1503 nt fragment of LINC00839; Red: 127–376 residues of β-catenin. E–G Deletion mapping for the domains of β-catenin that bind to LINC00839. RIP analysis for LINC00839 enrichment in cells transiently transfected with FLAG-tagged full-length or truncated constructs. H–J Immunoblotting of β-catenin in pull-down samples by full-length biotinylated-LINC00839 (#1), biotinylated-LINC00839 antisense (#2), or truncated biotinylated-LINC00839 RNA motifs (#3–10). K GSCs were transfected with FL or TL LINC00839. Representative images of spheres were photographed. Scale bars, 1 mm. L GSCs were transfected with FL or TL LINC00839. Cells were treated with or without IR (0–10 Gy) and seeded in 10 cm dishes. Colonies were counted after 2 weeks, and the surviving fraction was calculated as the ratio of the plating efficiency of the treated cells to that of control cells.