Fig. 2: SIRT3 downregulation mediates PDHA1 hyperacetylation and inactivation, leading to lactate overproduction. | Cell Death & Disease

Fig. 2: SIRT3 downregulation mediates PDHA1 hyperacetylation and inactivation, leading to lactate overproduction.

From: PDHA1 hyperacetylation-mediated lactate overproduction promotes sepsis-induced acute kidney injury via Fis1 lactylation

Fig. 2

A, B Changes in PDHA1 acetylation and PDH activity within 24 h after CLP in mice (n = 4). C, D Changes in PDHA1 acetylation and PDH activity in HK-2 cells within 24 h after LPS stimulation (n = 4). E, F Effects of SIRT3-specific inhibitor 3-TYP (5 mg/kg) treatment on CLP-induced PDHA1 hyperacetylation and PDH activity in mice (n = 5). G, H Effects of SIRT3 overexpression on LPS-induced PDHA1 hyperacetylation and PDH activity in HK-2 cells (n = 4). I, J Effects of SIRT3 knockdown on LPS-induced PDHA1 hyperacetylation and PDH activity in HK-2 cells (n = 4). K co-IP showing the interaction of SIRT3 with PDHA1 in HK-2 cells. L, M Effects of PDHA1 K385R mutation on PDHA1 acetylation and PDH activity in HK-2 cells (n = 4). N, O Effects of SIRT3 overexpression and SIRT3 knockdown on LPS-induced lactate production in HK-2 cells, respectively (n = 4). P Effects of PDHA1 K385R mutation on LPS-induced lactate production in HK-2 cells (n = 8). Data are mean ± SD; *P, #P < 0.05.

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