Fig. 8: The effects of reducing lactate and Fis1 lactylation on SAKI. | Cell Death & Disease

Fig. 8: The effects of reducing lactate and Fis1 lactylation on SAKI.

From: PDHA1 hyperacetylation-mediated lactate overproduction promotes sepsis-induced acute kidney injury via Fis1 lactylation

Fig. 8

A, B Effects of DCA (5 mM) treatment on Fis1 K20la level in LPS-stimulated HK-2 cells (n = 6). C, D Effects of SIRT3 overexpression on Fis1 K20la level in LPS-stimulated HK-2 cells (n = 6). E, F Effects of 3-TYP (50 μM) and GSK (5 μM) treatment on Fis1 K20la level in LPS-stimulated HK-2 cells (n = 4). G, H Effects of intraperitoneal injection of DCA (25 mg/kg) on SCr and BUN in CLP mice (n = 3). I Effects of intraperitoneal injection of DCA on renal pathological damage in CLP mice. Black arrow: nuclei of renal tubular cells shed to the lumen; White arrow: renal tubular vacuoles, structural disorder; White triangle: brush border loss; Red arrow: renal tubular basement membrane rupture. Scale bars, 50 μm. J Effects of intraperitoneal injection of 3-TYP (5 mg/kg) and GSK (20 mg/kg) on renal pathological damage in CLP mice. Scale bars, 50 μm. K Schematic of PDHA1 hyperacetylation-mediated lactate overproduction promoting SAKI via Fis1 lactylation. Data are mean ± SD; *P, #P < 0.05.

Back to article page