Fig. 5: Smurf1 or Smurf2 knockdown accelerates cell proliferation and tumor growth in Daoy cells. | Cell Death & Disease

Fig. 5: Smurf1 or Smurf2 knockdown accelerates cell proliferation and tumor growth in Daoy cells.

From: Smurf1 and Smurf2 mediated polyubiquitination and degradation of RNF220 suppresses Shh-group medulloblastoma

Fig. 5

A Growth curve for control, Smurf1 or Smurf2 knockdown Daoy cell line revealed by MTS assays. B, C EdU incorporation assays to evaluate DNA synthesis and proliferation rate of Daoy cells when Smurf1 or Smurf2 was knocked down. Scale bar, 50 µm. Quantification of EdU assay result was shown in (C). D–F Soft agar colony formation assays for the indicated Daoy cell line. Scale bar, 120 µm. Quantification of colony number and size was shown in (E, F). G–I Xenograft tumors from BALB/c nude mice subcutaneously injected with Daoy cells stably transfected with shRNAs against Smurf1 or Smurf2. Quantification of xenograft was shown in (H) for tumor size and (I) for tumor weight. J–N The mRNA (J, K) and protein expression (L–N) level of Shh targets (J, L, and M) and RNF220 (K, L, and N) in the indicated tumor xenografts. S1 Smurf1, S2 Smurf2, IB immunoblot, ns not significant p > 0.05, *p < 0.05 and **p < 0.01.

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