Fig. 2: LMO2 knockdown reduces cell proliferation, induce apoptosis, and suppresses the colony-forming capacity in AML cells.
From: LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1
A LMO2 knockdown in NB4, Kasumi-1, and K562 cells. LMO2 and GAPDH (loading control) protein expression. B Cell proliferation was performed by Cell Counting Kit 8 (WST-8/CCK-8) assay over a 4-day period in NB4, Kasumi-1, and K562 cells. C Apoptosis by Annexin V staining. (Right) Protein expression levels of PARP, CMYC, and GAPDH (loading control). D Microscopic changes of NB4, Kasumi, and K562 cells after LMO2 knockdown. E Colony numbers of NB4, Kasumi-1, and K562 cells after LMO2 knockdown in soft agar. Data are presented as mean ± sd. The colony experiments were performed independently twice times, other experiments were performed in triplicate.
