Fig. 3: LMO2 directly interacts with LDB1 in AML cells.
From: LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1
A The proteins identified by mass spectrometry were sorted according to the score of protein matching degree by Sequest HT. LDB1 was in the first place, and the number of peptides matched to the secondary map (PSMs) was also in the forefront. B The two-dimensional peak diagram shows one of the sequences of the peptide corresponding to the LDB1 protein. C NB4 cells expressing LMO2 were immunoprecipitated with HA antibody, and LDB1 protein was detected by WB. NB4 cells were immunoprecipitated with LDB1 antibody and LMO2 protein was detected by WB. D Expression of LDB1 protein in AML cell lines. E The LDB1 mRNA was detected by qRT-PCR in shRNA-transfected NB4, Kasumi-1, and K562 cells. F LDB1 and GAPDH (loading control) protein expression. G Microscopic cell morphology of NB4, Kasumi, and K562 cells after LDB1 interference. Data are presented as mean ± sd for at least three independent experiments.
