Fig. 6: Transcriptomic identification of potential targets of LDB1 in acute myeloid leukemia cells.
From: LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1
A RNA seq volcano plots showing changes in gene expression following LDB1 knockdown in NB4 cells. Red and blue indicate upregulated and downregulated genes, respectively. B GSEA analysis revealed the most abundant gene expression signatures in NB4 cells after LDB1 deletion. C Distribution of LDB1 binding to genomic regions in the NB4 cells, as assessed by ChIP-Seq. The pie chart showing the peak annotation of LDB1. D Heat map, analyzed from ChIP-Seq data, showing the occupancy of LDB1 in NB4 unit. Centering on LDB1 peak, this is further divided into TSS and non-TSS regions. E According to the ChIP-Seq, HOMER de novo motif analysis was then performed on the sequence data of LDB1 ChIP. The identified de novo motif was matched to a database of known motifs. F Comprehensive analysis for the identification of transcriptome-wide potential targets of LDB1 in acute myeloid leukemia by RNA-seq and ChIP-Seq results. G Validation of mRNA expression changes in potential gene targets of LDB1 by RT-PCR.
