Fig. 8: α-KG upregulates NR_033928 expression by promoting NR_03928 promoter demethylation. | Cell Death & Disease

Fig. 8: α-KG upregulates NR_033928 expression by promoting NR_03928 promoter demethylation.

From: m5C-methylated lncRNA NR_033928 promotes gastric cancer proliferation by stabilizing GLS mRNA to promote glutamine metabolism reprogramming

Fig. 8

A qRT-PCR analysis of NR_033928 expression in glutamine depletion medium in GC cells. B qRT-PCR analysis of NR_033928 expression in GC cells transfected with sh-GLS. C qRT-PCR analysis of NR_033928 expression in glutamine depletion medium in GC cells after DM-α-KG(10 µM 24h) treatment. D Schematic diagram of α-KG-dependent histone demethylation by JMJDs and DNA demethylation by TETs. E qRT-PCR analysis of NR_033928 expression in GC cells after GSK J4(4 µM 24h) treatment. F qRT-PCR analysis of NR_033928 expression in GC cells upon combined silencing of Tet1, Tet2 and Tet3. G, H hMeDIP assays of the relative hm5C methylation levels of NR_033928 promoter in GC cells in glutamine depletion medium treated with DM-α-KG(10 µM 24h) or combined with sh-Tet1-3. (Graph represents mean ± SD; **p < 0.01 and ***p < 0.001).

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