Fig. 5: Knockdown of Porf-2 facilitates the recovery of visual function after ONC injury.

A Representative individual B-scan images (scan 1 at 0° in en face images) from AAV2-shCtrl-injected and AAV2-shPorf-2-injected mice before ONC injury and 3 weeks after ONC injury. A vertical caliper was placed on each side of the optic nerve head, 500 µm away from the center of the optic nerve head. The caliper was used to determine the thickness of the ganglion cell complex (GCC), comprising the three innermost retinal layers: the nerve fiber layer (NFL), the ganglion cell layer (GCL), and the inner plexiform layer (IPL). Scale bar, 200 µm. B Representative pSTR amplitudes from mice injected with AAV2-shCtrl or AAV2-shPorf-2 before ONC injury and 3 weeks after ONC injury. The pSTR amplitudes were assessed at a flash intensity of 3 × 10^-5cd.sm^-2. The pSTR was measured from the baseline to the positive peak of the waveform. Scale bar, 10 μV, 100 ms. C Representative pupil changes from mice injected with AAV2-shCtrl or AAV2-shPorf-2 before ONC injury and 8 weeks after ONC injury. The contraction of the pupil is the area of the pupil before light exposure minus the area of the pupil after light exposure, divided by the area of the pupil before light exposure. D Quantification of the thickness of the GCC in (A) (Mann Whitney test, p < 0.01; n = 6 mice in each group). E Quantification of the pSTR amplitudes in (B) (Mann–Whitney test, p < 0.01; n = 6 mice in each group). F Quantification of the pupil changes in (C) (Mann–Whitney test, p < 0.0001; n = 6 mice in each group). Data are presented as the mean ± SEM. **p < 0.01, ****p < 0.0001.