Fig. 6: Knockdown of Porf-2 after ONC injury boosts RGC survival and axon regeneration. | Cell Death & Disease

Fig. 6: Knockdown of Porf-2 after ONC injury boosts RGC survival and axon regeneration.

From: Knockdown of Porf-2 restores visual function after optic nerve crush injury

Fig. 6

A Diagram of the experimental design. B Representative confocal images of optic nerves from Porf-2-knockdown and control RGCs two weeks after ONC injury. Asterisks indicate the optic nerve crush site. Scale bar, 200 μm. C Quantification of optic nerve regeneration in (B) (two-way ANOVA followed by Bonferroni’s multiple-comparisons test, p < 0.0001 at 0.2, 0.5, and 1.0 mm from the crush site; p < 0.01 at 1.5 mm from the crush site; n = 8 mice in each group). D Representative confocal images of retinal sections showing RBPMS+ RGCs (magenta) from Porf-2 knockdown and control mice two weeks after ONC injury. Scale bar, 20 μm. E Quantification of the RGC survival rate in (D) (Mann–Whitney test, p < 0.01; n = 6 mice in each group, at least eight non-adjacent retinal sections were analyzed for each retina). Data are presented as the mean ± SEM. **p < 0.01, ****p < 0.0001. ns, not significant.

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