Fig. 5: Inhibition of Plk1 prevented TGF-β1-induced fibroblast activation.
From: Plk1 promotes renal tubulointerstitial fibrosis by targeting autophagy/lysosome axis
In NRK49F cells, Plk1 was knocked down by Plk1-siRNA transfection and A–C Protein and mRNA level of Plk1 in control and Plk1 knockdown cells. D, E Western blot analysis of TGF-β1-induced FN and Collagen III expression. F Double-staining of α-SMA and phalloidin (F-actin) in response to TGF-β1 in different groups. Scale bar = 10 µm. For pharmacological inhibition of Plk1, NRK49F cells were treated with BI6727. G To determine cytotoxicity, LDH release in culture medium was analyzed at different concentrations of BI6727. H Western blot analysis of FN, Smad2 and p-Smad2 in cells treated with BI6727 in the presence of TGF-β1. All cell experiments were performed in triplicates. *P < 0.05, **P < 0.01, ***P < 0.001.
