Fig. 1: GSDMD-mediated pyroptosis is activated in epidermal keratinocytes of psoriasis lesion.

A H&E staining and expression of Ki-67 and N-GSDMD in normal skin tissue samples and skin lesion samples from psoriasis patients were shown. Red arrow indicates N-GSDMD. B Cleaved caspase-1 (green) and PI staining (red) were visualized by immunofluorescence assay. Nucleus was stained by Hoechst (n = 10). Red arrow indicates PI positive cells. C The cleavage levels of GSDMD and IL-1β were detected in epidermis lysate of normal skin tissue samples and skin lesion samples from psoriasis patients through western blotting assay. D WT mice (C57BL/6NGpt) were stimulated by imiquimod or vaseline, respectively. Skin appearances were presented. Histological features were analyzed by H&E staining. Immunohistochemistry study showed the levels of Ki-67. The severity of the lesions was evaluated by PASI scores. The epidermal thickness was measured by Image J software. n = 4. E The co-___location of GSDMD and cellular membrane was presented by immunofluorescence assay. Nucleus was stained by Hoechst. Actin filaments was stained by phalloidin (n = 4). F The cleavage levels of caspase-1, GSDMD and IL-1β were detected in epidermis lysate of mice through western blotting assay. G Flow cytometry was used to determine the level of keratinocyte death. Scale bar represents 100 μm in A. Scale bar represents 500 μm in B. Scale bar represents 50 μm in E. *p < 0.05, **p < 0.01, ***p < 0.001, WT wild type, GSDMD-FL GSDMD full length, N-GSDMD N-terminal GSDMD.