Fig. 4: keratinocyte-specific GSDMD knockout alleviated imiquimod induced psoriasis-like inflammation in mice.

Control (Krt14^+/+-Gsdmd^flox/flox) mice and Gsdmd cko (Krt14^Cre/+-Gsdmd^flox/flox) mice were stimulated by imiquimod or vaseline (A–H). A Skin appearances were presented. B The severity of the lesions was evaluated by PASI scores (for each group, n = 5). C The epidermal thickness was measured by ImageJ software (for each group, n = 5). D Histological features were analyzed by H&E staining. Immunohistochemistry study showed the levels of Ki-67 (n = 5). E The efficiency of keratinocyte-specific GSDMD knockout was confirmed by immunofluorescence assay. F Western blotting assay was used to detect the level of cleaved GSDMD and cleaved IL-1β in epidermis lysate of control or Gsdmd cKO mice. G The levels of IL-17 and TNF-α in peripheral blood of imiquimod-stimulated control mice or Gsdmd cKO mice were measured by ELISA (n = 3). H Ki-67+ and FVS 780+ epidermal cells from imiquimod-stimulated control mice or Gsdmd cKO mice were measured by flowcytometry (n = 3). Scale bar represents 100 μm in D. Scale bar represents 50 μm in E. *p < 0.05, **p < 0.01, ***p < 0.001. GSDMD-FL GSDMD full length, N-GSDMD N-terminal GSDMD.