Fig. 3: PRMT1-mediated BRD4 methylation regulates BRD4 phosphorylation.
From: Methylation of BRD4 by PRMT1 regulates BRD4 phosphorylation and promotes ovarian cancer invasion
a IB analysis of WCL and IP derived from HEK293T cells transfected with HA-BRD4 (1–470aa), Flag-PRMT1-WT and Flag-PRMT1-mutants (Flag-PRMT1-G80R, Flag-PRMT1-E144Q). EV empty vector. b IB analysis of WCL derived from HEK293T cells transfected with HA-PRMT1-WT and HA-PRMT1-G80R, HA-PRMT1-E144Q. c–e IB analysis of BRD4-S492/494 derived from HEK293T cells, SKOV3 cells, and A2780 cells treated with AMI-1 in a time-dependent manner for 24, 48, and 72 h. f and g IB analysis of WCL derived from SKOV3 cells and A2780 cells transfected with shPRMT1-1, shPRMT1-2, or shPRMT1-3. h IB analysis of BRD4-S492/494 derived from HEK293T cells transfected with Flag-PRMT1. i IB analysis of BRD4 derived from cell fractionations separated from PRMT1 conditional knockout SKOV3 cells. j and k Representative images and statistical analysis of A2780 cells infected with shPRMT1 lentivirus and stained for BRD4-S492/494 by IF. Scale bar, 100 μm. **P < 0.01.
