Fig. 7: PRMT1-mediated BRD4 methylation is correlated with TGF-β. | Cell Death & Disease

Fig. 7: PRMT1-mediated BRD4 methylation is correlated with TGF-β.

From: Methylation of BRD4 by PRMT1 regulates BRD4 phosphorylation and promotes ovarian cancer invasion

Fig. 7

a and b IB analysis of TGF-β derived from SKOV3 cells and A2780 cells transfected with shPRMT1. c and d IB analysis of TGF-β derived from SKOV3 cells and A2780 cells treated with AMI-1 for 24, 48, and 72 h. e–g IB analysis of TGF-β derived from SKOV3 cells, A2780 cells, 293T cells stably expressed HA-BRD4-WT and HA-BRD4-R3K mutant. h and i IB analysis of TGF-β derived from BRD4 knockdown 293T cells and BRD4 knockdown A2780 cells stably expressed HA-BRD4-WT and HA-BRD4-R3K mutant. j IB analysis of p-Smad2 and p-Smad4 derived from A2780 cells transfected with shPRMT1-1 and shPRMT1-2. k IB analysis of TGF-β derived from A2780 cells infected with sgTGF-β. l CCK8 assays were performed in TGF-β knockout A2780 ovarian cancer cells for 24, 48, and 72 h. Statistical analysis of relative colony numbers was plotted. ****P < 0.0001. m and n Colony formation were performed in TGF-β knockout A2780 cells. Statistical analysis of relative colony numbers was plotted. ***P < 0.001. o and p A2780-WT or TGF-β knockout (TGF-β KO) cells were injected into the subcutaneous of nude mice (n = 5 mice). The in vivo tumor size was monitored and tumors were dissected. ** P < 0.01. q CCK8 assays were performed in A2780 cells transfected with sh-PRMT1 and cells transfected with shPRMT1 and TGF-β for 24, 48, and 72 h.

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