Fig. 2: BEX2 interacts with NDP52 and LC3B to regulate mitophagy in NSCLC cells. | Cell Death & Disease

Fig. 2: BEX2 interacts with NDP52 and LC3B to regulate mitophagy in NSCLC cells.

From: Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells

Fig. 2

A Co-IP assays were carried out with the BEX2 antibody or IgG in H1299 cells followed by western blotting using anti-BEX2 and anti-NDP52 antibodies. B Schematic diagram of NDP52 and the ___domain-deleted constructs (top). Schematic diagram of BEX2; LIR, LC3-interacting region (bottom). C HEK293FT cells were co-transfected with MYC-NDP52 or MYC-tagged ___domain-deleted mutants of NDP52 together with CTRL (pcDNA3.1) or FLAG-BEX2, and then co-IP assays were carried out with an MYC antibody followed by western blotting using the indicated antibodies. D H1299 cells were transfected with CTRL (pcDNA3.1) or GFP-LC3, and then co-IP assays were carried out with GFP antibody followed by western blotting using anti-GFP and anti-BEX2 antibodies. E HEK293FT cells were transfected with FLAG-BEX2 or FLAG-tagged LIR-deleted mutants of BEX2 together with GFP-LC3B, and then co-IP assays were carried out with FLAG antibody followed by western blotting using anti-BEX2 and anti-GFP antibodies. F A549 cells were transfected with RFP-BEX2 and GFP-LC3, and then cells were pre-treated with BafilomycinA1(20 nM) and Liensinine (20 μM) for 0.5 h then incubated with CCCP (20 μM) for 6 h. MitoTracker Deep Red labeled mitochondria. The co-localization with BEX2, LC3 and mitochondria was monitored by confocal microscopy. G A549 cells were co-transfected with MYC-NDP52 and CTRL(pcDNA3.1) or BEX2, and then the cells were incubated with CCCP (20 μM) for 6 h. Cell lysates were incubated with an MYC antibody by co-IP assays followed by western blotting using the indicated antibodies.

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