Fig. 3: Overexpression of SIRT2 in renal tubular alleviated UUO-induced renal fibrogenesis.
From: SIRT2 alleviated renal fibrosis by deacetylating SMAD2 and SMAD3 in renal tubular epithelial cells
AAV-Ctrl or AAV-Ggt (gamma-glutamyltransferase 1)-Sirt2 was injected into bilateral kidneys of mice in situ at five independent points in situ. After 2-week transfection, the mice received UUO surgery, and the contralateral kidneys were used as control. a–d Western blot analysis of CTGF, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and β-actin in the kidneys from mice at day 10 post-surgery, with quantitative results shown in the right panel, and β-actin used as the loading control (n = 6). e Representative images of Sirius red staining, α-SMA immunohistochemical staining, and E-cadherin immunofluorescence staining were shown in the kidney from mice at day 10 post-surgery. The boxed areas in the upper panels are enlarged in the lower panels. The polarized distribution of E-cadherin in the basolateral membrane of tubules was indicated by white arrows; re-distribution of E-cadherin to the apical membrane of tubules is indicated by red arrows. Key in (b) also applies to (d). For all panels, the data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with a Bonferroni correction test.
