Fig. 4: LAIR1 induced nuclear FAK-dependent cyclin D1 expression.

A Immunohistochemical staining for LAIR1, FAK, p-FAK, and cyclin D1 in serial sections of the glioma tissues of mice bearing LAIR1OE or shLAIR1 GL261 cells (n = 6). B Immunofluorescence staining for FAK in LAIR1OE and shLAIR1 GL261 cells (n = 3). C Nuclear, cytoplasmic, and total FAK, SHP2, and cyclin D1 expressions in LAIR1OE and shLAIR1 GL261 cells by Western blot (a) and quantification of protein bands (b) (n = 3). D Effects of FAK^wt and FAK^NLM on cell proliferations of shLAIR1 GL261 cells by MTS assay (n = 3). E The transcriptional activities of the Ccnd1 promoter in LAIR1OE or shLAIR1 GL261 cells by luciferase reporter assay (n = 5). F The transcriptional activities of the Ccnd1 promoter in shLAIR1 GL261 cells co-transfected with FAK^wt and FAK^NLM (n = 5). G, H FAK and cyclin D1 expressions in the nuclear and total cells of shLAIR1 GL261 cells co-transfected with FAK^wt and FAK^NLM by Western blot (a) and quantification (b) (n = 4). The data were expressed as average ±SD. P values were labeled in each figure.