Fig. 6: LAIR1 induced CCL5 production and modulated microglia/macrophage polarization.

A Single-cell RNA sequencing analysis of CCL5 and TGFβ2 expression in GSE162631 cohort. B Single-cell RNA sequencing analysis of CCL5 and TGFβ2 expression in GSE138794 cohort. C Spearman correlation analysis between LAIR1 and microglia/macrophage makers of glioblastoma samples from the GTEx database (http://xena.ucsc.edu/public/) by R (version 4.1.2). D Representative images of LAIR1OE or shLAIR1 implanted tissues stained for binding of CD11b (red), CD163 (pink), and CD80 (green) antibodies. DAPI (blue) was used for nuclear staining. Scale bars: 50 μm (n = 3). E FACS quantification for percentage of CD163⁺/CD11b⁺ and CD 80⁺/CD11b⁺ in LAIR1OE or shLAIR1 implanted tissues (n = 3). F Relative mRNA expression levels of Il10, Tgfβ1, Il12, and Tnfα in BV2 cells cultured in the medium of LAIR1OE or shLAIR1 GL261 cells in the presence of CCL5 (10 ng/mL) or CCR5 antagonist TAK-779 (1.2 nM) for 48 h by RT-PCR (n = 5). The data were expressed as average ±SD. P values were labeled in each figure.