Fig. 5: SIRT4 mediated oxidative stress in SAP in AR42J cells.
From: Sirtuin4 alleviates severe acute pancreatitis by regulating HIF-1α/HO-1 mediated ferroptosis
A Western blot showing the SIRT4 knock down efficiency. B Transfection efficiency of SIRT4 overexpressed using the Flag plasmid in AR42J cells. C Viability of AR42J cells. D–E The levels of oxidative stress (GSH, SOD and MDA) in AR42J cells. F–G Representative images showing ROS in the AR42J cells. Scale bar = 100 µm. CON group (PBS), SIRT4 KD group (SIRT4 knockdown), CON + L-Arg group (10 mg/ml L-Arg), SIRT4 KD + L-Arg group (SIRT4 KD + 10 mg/ml L-Arg), SIRT4 OE group (SIRT4 overexpression), SIRT4 OE + L-Arg group (SIRT4 OE + 10 mg/ml L-Arg). A used GAPDH as the reference protein. *P < 0.05, **P < 0.01, ***P < 0.001, ns. not significant. n = 6 per group.
