Fig. 8: The regulatory effect of SIRT4 on ferroptosis in SAP depends on the HIF-1α/HO-1 pathway.
From: Sirtuin4 alleviates severe acute pancreatitis by regulating HIF-1α/HO-1 mediated ferroptosis
A–B The protein expression levels of HIF-1α, HO-1 and NQO-1 in the pancreas of mice. C Serum amylase level and lipase activity of mice in different groups after using HIF-1α inhibitor PX478 (100 mg/kg). D Representative HE staining images of mice pancreas in different groups after using HIF-1α inhibitor PX478 (100 mg/kg). Scale bar = 100 µm. E The levels of oxidative stress factors (GSH, H2O2, SOD, MDA) in the pancreas of mice in different groups after using HIF-1α inhibitor PX478 (100 mg/kg). F Representative IHC staining of CD11b, MPO, Ly6G and 4-HNE in the pancreas of mice in different groups after using HIF-1α inhibitor PX478(100 mg/kg). Scale bar = 100 µm. G The protein expression level of HIF-1α, HO-1, NQO-1, ACSL, GPX4 and SLC7A11 in the pancreas of mice in different groups after using HIF-1α inhibitor PX478 (100 mg/kg). WT + SAP group, SIRT4_KO + SAP group, SIRT4_KO + PX478 + SAP group. A–G used GAPDH as the reference protein. *P < 0.05, **P < 0.01, ***P < 0.001, ns. not significant. n = 6 per group.
