Fig. 7: NRF2 pathway was involved in the cytoprotection of SFN against IL-22 and TNF-α. | Cell Death & Disease

Fig. 7: NRF2 pathway was involved in the cytoprotection of SFN against IL-22 and TNF-α.

From: Sulforaphane alleviates psoriasis by enhancing antioxidant defense through KEAP1-NRF2 Pathway activation and attenuating inflammatory signaling

Fig. 7

a The expression of NRF2 and p-NRF2 in response to SFN and IL-22 treatment was determined by western blotting. b The expression of NRF2 and p-NRF2 in response to SFN and TNF-α treatment was determined by western blotting. c HaCaT cell superoxide measured by staining with DHE (magnification × 400). d HaCaT cells were transduced with lentivirus carrying shRNA against a scramble sequence (sh-C) or against NRF2(sh-NRF2-a and sh-NRF2-b). Expression levels of NFE2L2 were determined by qRT–PCR and normalized to Actb levels. e The Western Blot results of SFN and IL-22 treatment in the NRF2 knockdown group (sh-NRF2-b) and the control group (sh-C). f The Western Blot results of SFN and TNF-α treatment in the NRF2 knockdown group (sh-NRF2-b) and the control group (sh-C). *p < 0.05, **p < 0.01 and ***p < 0.001.

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