Fig. 3: Upregulating ACSL4 is involved in LSD1 inhibitor-induced ferroptosis. | Cell Death & Disease

Fig. 3: Upregulating ACSL4 is involved in LSD1 inhibitor-induced ferroptosis.

From: Inhibition of LSD1 induces ferroptosis through the ATF4-xCT pathway and shows enhanced anti-tumor effects with ferroptosis inducers in NSCLC

Fig. 3

A The expression of ACSL4 was examined in NSCLC cells treatment with 10 μM ORY for 96 h. B ACSL4 expression was determined by western blotting in A549 and H1975 cell lines treated with 10 μM GSK, 10 μM GSK-2879552, or 10 μM ORY for 96 h as indicated. C ACSL4 expression was determined by western blotting in A549 and H1975 cell lines treated with increasing concentrations of ORY for 96 h. D ACSL4 mRNA was determined by qRT-PCR in A549 and H1975 cell lines treated with increasing concentrations of ORY for 96 h. E The expression of LSD1 and ACSL4 was analyzed by western blotting in A549 and H1975 cell lines transfected with ACSL4 siRNA for 48 h. F–H After A549 and H1975 cell lines transfected with ACLS4 siRNA and were treated with 10 μM ORY for 96 h, ACSL4 expression (F) was determined by western blotting, cell viability (G) was detected by SRB, and the production of lipid peroxides (H) was analyzed by flow cytometry. I Quantitative ChIP studies were conducted to characterize the enrichment of H3K4me2 and H3K9me2 at promoters of ACSL4 gene in A549 cells treated with 10 μM ORY for 96 h. n ≥ 3; Mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.

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