Fig. 4: Decreased xCT is critical for LSD1 inhibitor to induce ferroptosis. | Cell Death & Disease

Fig. 4: Decreased xCT is critical for LSD1 inhibitor to induce ferroptosis.

From: Inhibition of LSD1 induces ferroptosis through the ATF4-xCT pathway and shows enhanced anti-tumor effects with ferroptosis inducers in NSCLC

Fig. 4

A The levels of GSH were detected in NSCLC cell lines treated with 10 μM GSK for 96 h. B, C After A549 and H1975 cell lines were treatment with 10 μM ORY for 48 h and 5 mM NAC or 1 mM DTT was added again for 48 h as indicated, the levels of GSH (B) and cell viability (C) were measured. D xCT expression was determined by western blotting in NSCLC cell lines treated with 10 μM ORY for 96 h. E The expression of xCT and LSD1 was analyzed by western blotting in A549 and H1975 cell lines transfected with LSD1 siRNA for 48 h. F xCT expression was analyzed by western blotting in A549 and NCI-H1975 cell lines transfected with LSD1 siRNA for 48 h. G, H After A549 and H1975 cell lines were transfected with xCT siRNA for 48 h and 5 mM NAC or 1 mM DTT was added again for 48 h as indicated, the levels of GSH (G) and cell viability (H) were measured. I xCT expression was analyzed by western blotting in xCT-overexpressing cells. J After xCT-overexpressing cells were treated with 10 μM ORY for 48 h and 5 mM NAC or 1 mM DTT was added again for 48 h as indicated, the levels of GSH were measured. K Cell viability was determined by SRB in xCT-overexpressing cells treated with ORY at increasing concentrations for 96 h. n ≥ 3; Mean ± SEM; nsP > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.

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