Fig. 6: Twist1 regulates adipocyte differentiation via inhibiting PPARĪ³ signaling.
From: Deciphering the interaction between Twist1 and PPARĪ³ during adipocyte differentiation
A Transcriptional regulation of PPARĪ³ or adiponectin by Twist1 was detected using dual luciferase reporter assay. pGL3-Basic: the empty vector control. B ChIP assay detected the binding sites of Twist1 to PPARĪ³ or Adiponectin promoter. PR-II: RNA polymerase, positive control and IgG: negative control. C Overexpression of PPARĪ³ was assessed by WB in Twist1-OE 3T3-L1 cells after being transfected with pcDNA3.1(+)-PPARĪ³ plasmids. pcDNA3.1(+): empty vector control. D Effect of Twist1 overexpression on the transcriptional activity of PPARĪ³ was examined with or without rosiglitazone by dual luciferase reporter assay. E Nuclear and cytoplasmic extraction of Twist1-OE 3T3-L1 cells at differentiation day 8. Lamin B1: nuclear marker and Ī²-Tubulin: cytoplasm marker. F PPARĪ³ transcriptional activity was measured in Twist1-OE 3T3-L1 cells at differentiation day 8 by ELISA after nuclear-cytoplasmic separation. The x-axis represents the concentration of the extracted nuclear protein in Āµg.
