Fig. 3: Ruxo-induced ATC cells apoptosis and pyroptosis. | Cell Death & Disease

Fig. 3: Ruxo-induced ATC cells apoptosis and pyroptosis.

From: Ruxolitinib induces apoptosis and pyroptosis of anaplastic thyroid cancer via the transcriptional inhibition of DRP1-mediated mitochondrial fission

Fig. 3

A Flow cytometry was used to measure cell deaths of 8505 C and KHM-5M after Ruxo (0, 40 and 80 μM) treatment for 24 h. B Characteristic morphology of 8505 C and KHM-5M cells induced by Ruxo (0, 40 and 80 μM) (apoptotic cells are shown by bule arrows while pyroptotic cells are shown by red arrows). C Relative LDH activities in the lysate of cells were measured by the LDH assay Kit. D Western blot was used to measure caspase 9, caspase 3, c-caspase 3, PARP, c-PARP, full-length GSDME and GSDME-N terminus protein levels in 8505 C and KHM-5M cells, after Ruxo (0, 40 and 80 μM) treatment for 24 h. Data are shown as mean ± SD for n = 3, analyzed by one-way ANOVA using the Holm-Sidak method (A and C) *p < 0. 05, **p < 0. 01, ***p < 0. 001, ****p < 0. 0001.

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