Fig. 8: DRP1 expression is closely associated with Ruxo-induced ATC cell death. | Cell Death & Disease

Fig. 8: DRP1 expression is closely associated with Ruxo-induced ATC cell death.

From: Ruxolitinib induces apoptosis and pyroptosis of anaplastic thyroid cancer via the transcriptional inhibition of DRP1-mediated mitochondrial fission

Fig. 8

Cell deaths (A), relative LDH activities in culture mediums (B), morphological alterations (C) and DRP1, caspase 3, c-caspase 3, PARP, c-PARP, full-length GSDME and GSDME-N terminus protein levels (D) of Ruxo -treated ATC cells (8505C, 40 μM and KHM-5M, 20 μM, for 24 h) with or without transfection of DRP1 overexpression plasmid were measured by flow cytometry, LDH assay and western blot. Cell deaths (E), relative LDH activities in culture mediums (F), morphological alterations (G) and DRP1, caspase 3, c-caspase 3, PARP, c-PARP, full-length GSDME and GSDME-N terminus protein levels (H) of ATC cells treated with or without Ruxo (8505C, 40 μM and KHM-5M, 20 μM, for 24 h) and Mdivi-1 (a DRP1 inhibitor), were measured by flow cytometry, LDH assay and western blot. (I and J) Tumor volumes were measured every other day, growth curves of tumors treated with Ruxo (15 mg/kg, intraperitoneal injection), Mdivi-1 (15 mg/kg, intraperitoneal injection) and Ruxo (15 mg/kg) +Mdivi-1 (15 mg/kg) (n = 6/group) were analyzed by GraphPad Prism 9 software. Data are shown as mean ± SD. K Representative gross image of tumors at the endpoint of the experiment were performed of each group. Data are shown as mean ± SD for n = 3, analyzed by two-way ANOVA using the Tukey method (A, B, E, F) for multiple comparisons. *p < 0. 05, **p < 0. 01, ***p < 0. 001, ****p < 0. 0001.

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