Fig. 3: MUC1-C suppresses expression of the YTHDF2 and CNOT1 proteins that destabilize m6A-containing RNAs.
From: XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression
a DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for expression of the indicated genes. The results (mean ± SD of three determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1). Lysates from DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days (b) and DU-145/CshRNA and DU-145/MUC1shRNA#2 (c) cells were immunoblotted with antibodies against the indicated proteins. d DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for CNOT1 mRNA levels. The results (mean ± SD of three determinations) are expressed as relative transcript levels compared to that obtained for vehicle-treated cells (assigned a value of 1). e Lysates from DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. f Lysates from DU-145 cells expressing the indicated vectors were treated with vehicle or DOX for 7 days and immunoblotted with antibodies against the indicated proteins.
