Fig. 6: MUC1-C/XIST pathway promotes the CSC state.
From: XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression
a DU-145 cells grown as 2D monolayers and 3D tumorspheres were analyzed for the indicated RNA levels. The results (mean ± SD of three determinations) are expressed as relative levels compared to that obtained for 2D cells (assigned a value of 1). b DU-145 3D/tet-MUC1-CshRNA cells treated with vehicle or DOX for 7 days were analyzed for XIST RNA levels. The results (mean ± SD of three determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1). c DU-145 3D/CshRNA and DU-145 3D/XISTshRNA cells were analyzed for the indicated mRNA levels. The results (mean ± SD of three determinations) are expressed as relative mRNA levels compared to that obtained for CshRNA-expressing cells (assigned a value of 1)(left). Lysates were immunoblotted with antibodies against the indicated proteins (right). d DU-145 3D/CshRNA and DU-145 3D/XISTshRNA were analyzed for expression of the indicated genes. The results (mean ± SD of three determinations) are expressed as relative mRNA levels compared to that obtained for CshRNA-expressing cells (assigned a value of 1)(left). Lysates were immunoblotted with antibodies against the indicated proteins (right). e DU-145 3D/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for expression of the indicated genes. The results (mean ± SD of three determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1)(left). Lysates were immunoblotted with antibodies against the indicated proteins (right). f DU-145 3D/CshRNA and DU-145 3D/XISTshRNA were analyzed for tumorsphere formation. Photomicrographs are shown for the tumorspheres (bar represents 100 μm; left). The results (mean ± SD of three determinations) are expressed as tumorsphere number(right). g, h. DU-145 3D cells expressing the indicated vectors were treated with vehicle or DOX for 7 days and analyzed for tumorsphere formation. Photomicrographs are shown for the tumorspheres (left). The results (mean ± SD of three determinations) are expressed as tumorsphere number (right).
