Fig. 6: TFAP2B transcriptionally regulates S100A16 expression in HK-2 cells.
From: HIF-1α participates in the regulation of S100A16-HRD1-GSK3β/CK1α pathway in renal hypoxia injury
A Western blots of TFAP2B and S100A16 in HK-2 cells overexpressed by TFAP2B. B Quantitation of western blot data for TFAP2B and S100A16 in A. **P < 0.01, *P < 0.05. C The transcriptional expressions of TFAP2B and S100A16 were tested by real-time PCR in HK-2 cells overexpressed by TFAP2B. **P < 0.01, *P < 0.05. D Analysis of the potential binding sites of the S100A16 promoter with TFAP2B according to the jaspar website. E ChIP assay was performed to determine the binding of TFAP2B to the S100A16 promoter. ****P < 0.0001, *P < 0.05; vs IgG. F Luciferase activity analysis in HK-2 cells transfected with different reporter luciferase plasmids. ****P < 0.0001, **P < 0.01; vs. PGL3. G Western blots of TFAP2B, S100A16, HIF-1α and HRD1 in S100A16−/− cells with or without TFAP2B overexpression. H Quantitation of western blot data for TFAP2B, S100A16, HIF-1α, and HRD1 in G. ****P < 0.0001, **P < 0.01.
