Fig. 1: CBX4 promotes the proliferation of LUAD cells in vitro.
From: CBX4 plays a bidirectional role in transcriptional regulation and lung adenocarcinoma progression
A CBX4 was knocked down by two independent short hairpin RNAs (shRNAs) in A549 and H1299 cells. Knockdown efficiency was assessed by Western blotting. The statistical results of the western blotting assays are presented in a bar chart. B MTT assays were performed in A549 and H1299 cells expressing control or CBX4 shRNAs. C A549 and H1299 cells stably expressing control or CBX4 shRNAs were cultured for 2 weeks, and stained with crystal violet. The number of colonies was counted. D A549 and H1299 cells expressing control or CBX4 shRNAs were subjected to EdU‐incorporation assays, and the percentage of EdU‐positive cells was calculated. Scale bar, 100 μm. E Cell lysate from A549 and H1299 cells stably expressing empty vector or Flag-CBX4 was subjected to Western blotting using indicated antibodies. F MTT assays were conducted in A549 and H1299 cells with or without CBX4 overexpression. G Colony formation assays were performed in indicated cells. H EdU-incorporation assays were conducted in A549 and H1299 cells with CBX4 overexpression or not. Scale bar, 100 μm. In figures A–D and F–H, each bar represents the mean ± SD for n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, indicating shCBX4 versus control, Flag-CBX4 versus vector (Student’s t-test).
