Fig. 5: CBX4 inhibits the migration and invasion of LUAD cells by suppressing the transcription of ZEB2.
From: CBX4 plays a bidirectional role in transcriptional regulation and lung adenocarcinoma progression
A Western blot analysis of ZEB2, CBX4, and β-actin expression in A549 cells expressing control or CBX4 shRNAs. B ChIP-qPCR assays were performed using anti-CBX4 or IgG, with primer pairs targeting the ZEB2 promoter in A549 cells. C Genome browser view of the H2AK119ub signal on the ZEB2 gene obtained by ChIP-seq in A549 cells. D ChIP-qPCR assays were performed using anti-H2AK119ub or IgG, along with primer pairs targeting the ZEB2 promoter in A549 cells expressing control and shCBX4-1. For figures B and D, each bar represents the mean ± SD for n = 3; **P < 0.01 versus control (Student’s t-test). E A549 cells stably expressing control shRNA or CBX4 shRNA-1 were transfected with ZEB2 siRNAs or not. The mRNAs were extracted, and subjected to real-time quantitative RT-PCR assay. F Wound-healing assays were performed using indicated cells. G Transwell assays were performed using A549 cells expressing control shRNA, shCBX4-1, and cells expressing both shCBX4-1 and siZEB2-2. Scale bars, 100 μm. For figures E-G, each bar represents the mean ± SD for n = 3; *P < 0.05, ***P < 0.001 (one-way ANOVA followed by Bonferroni post-hoc test or Tambane’s T2 post-hoc test).
