Fig. 4: Inhibition of PGC1A results in lipid droplet accumulation in ARPE 19 cells. | Cell Death & Disease

Fig. 4: Inhibition of PGC1A results in lipid droplet accumulation in ARPE 19 cells.

From: PGC-1α repression dysregulates lipid metabolism and induces lipid droplet accumulation in the retinal pigment epithelium

Fig. 4

ARPE19-PGC1A KO cells and Scramble control cells were treated with 2 μM doxycycline for 14 days to induce the CRISPR-Cas9 gene editing process. Lipid droplets were stained by LipidSpot 488 in both ARPE19-PGC1A KO and Scramble control groups subjected to treatment with medium containing 15% FBS for 6 weeks (A) and 10 weeks (B), respectively. The tight junction protein ZO-1 was stained in red, and nuclei were stained in blue by DAPI. The scale bar is 100 μM. C Relative quantification of lipid droplets in ARPE19-Scramble and ARPE19-PGC1A KO cells at 6 weeks and 10 weeks post-incubation with media containing 15% FBS. Unpaired t-test was performed for statistical analysis. Graph represents mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, *** P ≤ 0.001, ****P ≤ 0.0001, n.s. means no significance.

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