Fig. 9: Forced expression of BNC2 dampens OGTi-mediated decrease in collagen-encoding gene expression in MF-HSCs.
From: O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in myofibroblasts
A LX-2 cells were transfected with a control plasmid (mock) or 3x-FLAG-BNC2 encoding plasmid for 24 h. Then, cells were treated or not with 50 µM OSMI-1 (referred to as OGTi) for an additional 24 h and used to prepare extracts analyzed using western blot and simple western immunoassays. BNC2 levels were assessed and HSP90 was used as a protein loading control. The presented images are representative of 3 biologically independent experiments. MW, molecular weight markers. B LX-2 cells were transfected as in A and used for RT-qPCR analyses of indicated gene expression (n = 3 biologically independent experiments). The bar graphs show means + SD together with individual biological replicates. Log2 FC between OGTi and control conditions are shown. Unpaired t-test was used to assess the statistical significance between 3x-FLAG-BNC2 expressing cells and the control (mock) condition.
