Fig. 4: Upregulated ATF4-SPHK1 signaling in GBM cell in response to TMZ. | Cell Death & Disease

Fig. 4: Upregulated ATF4-SPHK1 signaling in GBM cell in response to TMZ.

From: Triggering of endoplasmic reticulum stress via ATF4-SPHK1 signaling promotes glioblastoma invasion and chemoresistance

Fig. 4

A Protein levels of ATF4 by western blotting analysis in LN229 and LN229ATF4O cells with TMZ treatment for 3 days. B Analysis of SPHK1 promoter activity using a dual-luciferase assay in LN229 and LN229ATF4O cells with TMZ treatment for 3 days. C mRNA levels of SPHK1 and ATF4 by qPCR in LN229-siATF4-2 cells and LN229 cells with TMZ treatment for 3 days. D Protein levels of SPHK1 and ATF4 by western blotting analysis in LN229-siATF4-2 cells and LN229 cells with TMZ treatment. E Protein levels of phosphorylated SPHK1 by western blotting analysis in LN229 and LN229ATF4O cells with TMZ treatment for 3 days. F Protein levels of phosphorylated SPHK1 by western blotting analysis in LN229-siATF4-1 cells, LN229-siATF4-2 cells, and LN229 cells with TMZ treatment for 3 days. G Immunofluorescent staining detection of SPHK1 expression and subcellular localization in LN229-siATF4-2 cells and LN229 cells treated with TMZ for 3 days (bar = 60 μm). (*p < 0.05, **p < 0.01, ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001).

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