Fig. 5: Targeting SPHK1 inhibits GBM cell aggression and TMZ resistance. | Cell Death & Disease

Fig. 5: Targeting SPHK1 inhibits GBM cell aggression and TMZ resistance.

From: Triggering of endoplasmic reticulum stress via ATF4-SPHK1 signaling promotes glioblastoma invasion and chemoresistance

Fig. 5

A Western blotting analysis for silencing SPHK1 in LN229 and U87MG cells. B MTT assay to detect cell viability in LN229 and U87MG cells with TMZ treatment for 3 days. C Flow cytometry detected apoptosis in LN229 cells with TMZ treatment for 3 days. D, E Wound healing assay detected the migration of LN229 and U87MG cells treated with TMZ for 24 h and 48 h under serum-free culture conditions. F, G Trans-well assay to detect the invasion ability of LN229 and U87MG cells treated with TMZ for 12 h. (*p < 0.05, **p < 0.01, ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001).

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