Fig. 2: scRNA-seq identifies trauma-specific immune-cell states and gene signatures.
From: The different paradigms of NK cell death in patients with severe trauma
a Schematic diagram detailing trauma single-cell sequencing cohort construction. b Eleven distinct blood immune cells were numbered, named, and displayed with a uniform manifold approximation and projection (UMAP) plot. Each point represents a single-cell colored by its cell type. c Canonical markers of each cell type were plotted using a heatmap. Color scale corresponds to z-scored, log-transformed mean gene-expression counts for each cell type. d Proportion of each immune population out of all immune cells in (a). Each block of the bar is colored by its cell type, and the number indicates the percentage of NK cells at each time point after injury. p values were calculated by comparing them with healthy controls using a two-tailed Wilcoxon rank-sum test. *p < 0.05; e UMAP plots for each post-trauma time point (n = 34423, 19195, 31752, 18380, and 19195 cells for control, post-trauma 4 h, post-trauma 6 h, post-trauma 24 h and post-trauma 72 h, respectively), colored and labeled by cell types. f Cell death gene set enriched in the post-trauma 4, 6, 24, and 72 h patients compared to the healthy controls. g Bubble plot of cell death gene set normalized enrichment score (NES) across different time points compared to healthy controls. Different groups colored each bubble. DC dendritic cell, NK natural killer, HSC hematopoietic stem cell, GO Gene ontology.
