Fig. 3: APOH increased the fatty acid and cholesterol biosynthesis pathway.

A Scheme for the procedure of MEFs’ original cultured medium that was exchanged for 12 h or 24 h or remain unchanged. R-Scm 12 h, R-Scm 24 h, MEFs culture medium changed from Rcm to Scm for 12 h or 24 h; S-Rcm 12 h, S-Rcm 24 h, MEFs cultured medium changed from Scm to Rcm for 12 h or 24 h. B KEGG pathway enrichment analysis for differentially expressed genes (DEGs) altered by the culture medium exchanged. C, D Transcription factor analysis of DEGs in MEFs after changing the medium. The transcription factors of MEFs activated by changing the medium from Scm to Rcm for 24 h (C) and suppression by changing the medium from Rcm to Scm for 24 h (D). E Western blot analysis of the protein levels of SREBP1 in MEFs cultured in Scm and in cells in which the medium was changed from Scm to Rcm for the indicated time. p-SREBP1, precursor SREBP1. m-SREBP1, mature SREBP1. S, short exposure; L, long exposure. F the mRNA level of the indicated SREBP1 target genes of MEFs cultured in Scm or Rcm by RT-qPCR. G mRNA level of the indicated SREBP1 target genes of MEFs cultured in Scm changed medium to 5% Scm + 5% Rcm (containing 5% S-FBS and 5% R-FBS) or 5% Scm + APOH (containing 5% S-FBS and 120 μg/mL APOH) for 12 h by RT-qPCR. Data are presented as mean ± s.d., n = 3 independent repeats. Unpaired, two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NS not significant.