Fig. 8: VCP-USP2-FASN axis promotes OS progression in vivo. | Cell Death & Disease

Fig. 8: VCP-USP2-FASN axis promotes OS progression in vivo.

From: VCP enhances autophagy-related osteosarcoma progression by recruiting USP2 to inhibit ubiquitination and degradation of FASN

Fig. 8

A Representative images of xenograft tumors after established by subcutaneous injection of 143B cells stably transfected with Ca-NC+Ci-NC, Ca-VCP, Ca-VCP+Ci-FASN or Ca-VCP+Ci-USP2 for 2 weeks (left). Quantification of volume and weight of tumor nodules in indicated groups(right). B Representative micro-CT images were obtained from in situ tumors after injection of 143B cells stably transfected with Ca-NC+Ci-NC, Ca-VCP, Ca-VCP+Ci-FASN, or Ca-VCP+Ci-USP2 into the proximal tibia for 3 weeks. C, D HE, IHC and quantitative analyses in subcutaneous (left) and orthotopic (right) xenograft models revealed the tumor cells and the differences in FASN, VCP, and USP2 protein expression. Scale bar: 50 μm. E Mechanisms underlying FASN-promoted autophagy and OS progression. *P < 0.05.

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