Fig. 1: ZBP1 mediates DNA damage-induced cell death. | Cell Death & Disease

Fig. 1: ZBP1 mediates DNA damage-induced cell death.

From: Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy

Fig. 1

a WT or ZBP1−/− MDFs were treated with ETO (50 μM), MNNG (500 μM), CDDP (50 μM) or 5-FU (50 μg/mL) for 16 h and representative merged images of phase-contrast and PI staining were shown. Scale bar, 100 μm. b Cell death of the MDFs in (a) was determined by PI staining. c MDFs were treated with ETO, d MNNG or e CDDP at the indicated time points. Cells were lysed and immunoblotted with the indicated antibodies. hrs, hours. f WT, ZBP1−/− or ZBP1 reconstituted MDFs were treated with ETO or MNNG for 16 h, and cell death was determined by PI staining. g WT, ZBP1−/− or ZBP1 reconstituted MDFs were treated with ETO or h MNNG at the indicated time points. Cells were lysed and immunoblotted with the indicated antibodies. Data are from at least three independent experiments. Bar graphs represent the mean ± SD from three independent experiments. Statistical analysis was performed using a two-sided Student’s t-test. NS no significant (P > 0.05); *P < 0.05; **P < 0.005 (two-way ANOVA).

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