Fig. 2: DNA damage induces PANoptosis by promoting ZBP1-PANoptosome assembly. | Cell Death & Disease

Fig. 2: DNA damage induces PANoptosis by promoting ZBP1-PANoptosome assembly.

From: Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy

Fig. 2

a WT or ZBP1−/− MDFs were treated with ETO at the indicated time points. Cells were lysed and immunoblotted with the indicated antibodies. b sgRNA-Control, sgRNA-RIPK3#1 and sgRNA-RIPK3#2 MDFs were treated with vehicle control or ETO for 16 h and cell death was determined by PI staining. c Cells in (b) were lysed and immunoblotted with the indicated antibodies. d WT or ZBP1−/− MDFs were treated with ETO at the indicated time points. Cells were lysed and immunoblotted with phospho-RIPK3 and RIPK3 antibodies. e sgRNA-Control, sgRNA-Casp6#1 and sgRNA-Casp6#2 MDFs were treated with vehicle control or ETO for 16 h and cell death was determined by PI staining. f Cells in (e) were lysed and immunoblotted with the indicated antibodies. g ZBP1 reconstituted MDFs were treated with ETO for 12 h. Cell lysates were immunoprecipitated with FLAG antibody (IP: FLAG) and analyzed by immunoblotting with indicated antibodies. Data are from at least three independent experiments. Bar graphs represent the mean ± SD from three independent experiments. Statistical analysis was performed using a two-sided Student’s t-test. NS not significant (P > 0.05); *P < 0.05; **P < 0.005 (two-way ANOVA).

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