Fig. 3: The Zα ___domain of ZBP1 is required for DNA damage-induced PANoptosis. | Cell Death & Disease

Fig. 3: The Zα ___domain of ZBP1 is required for DNA damage-induced PANoptosis.

From: Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy

Fig. 3

a Schematic representation of full-length ZBP1. ZBP1 encodes two N-terminal Zα domains and two C-terminal RHIM domains. b WT or ZBP1mutZα1α2 MDFs were treated with ETO or MNNG for 16 h, and representative merged images of phase-contrast and PI staining were shown. c Cell death of the MDFs in (b) was determined by PI staining. d Cells in (b) were treated with ETO or e MNNG for 16 h. Cells were lysed and immunoblotted with the indicated antibodies. f Flag-ZBP1 or Flag-ZBP1mutZα1α2 were ectopically expressed in ZBP1−/− MDFs and treated with ETO for 12 h, respectively. Cell lysates were immunoprecipitated with FLAG antibody (IP: FLAG) and analyzed by immunoblotting with indicated antibodies. Data are from at least three independent experiments. Bar graphs represent the mean ± SD from three independent experiments. Statistical analysis was performed using a two-sided Student’s t-test. NS not significant (P > 0.05); *P < 0.05; **P < 0.005 (two-way ANOVA).

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