Fig. 4: ZBP1 binds dsRNA to promote PANoptosome assembly in DNA damage. | Cell Death & Disease

Fig. 4: ZBP1 binds dsRNA to promote PANoptosome assembly in DNA damage.

From: Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy

Fig. 4

a ZBP1 reconstituted MDFs were treated with ETO for 12 hrs. Cells were lysed and treated with DNase I or RNase A for 1 h. Cell lysis was then immunoprecipitated with FLAG antibody (IP: FLAG) and analyzed by immunoblotting with indicated antibodies. b WT MDFs were treated with ETO for 12 h and then exposed to RNase III, RNase A or DNase I for 1 h, respectively. Cells were stained with anti-dsRNA J2 antibody and detected by immunofluorescence. c WT MDFs were treated with ETO for 12 h or CBL0137 (5 μM) for 4 h. Cells were stained with anti-Z-NA Z22 antibody and detected by immunofluorescence. d ZBP1-reconstituted MDFs were treated with ETO for 12 h. The physical associations between FLAG-ZBP1 and dsRNA were detected as red spots using PLA assay. Scale bar, 20 μm. Data are from at least three independent experiments.

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