Fig. 4: ER stressor-induced miR-378high FLSs promoted angiogenesis and osteoclastogenesis. | Cell Death & Disease

Fig. 4: ER stressor-induced miR-378high FLSs promoted angiogenesis and osteoclastogenesis.

From: MiR-378 exaggerates angiogenesis and bone erosion in collagen-induced arthritis mice by regulating endoplasmic reticulum stress

Fig. 4

A Diagram to show the collection of conditioned medium from RA-FLSs and the co-culture system for angiogenesis and osteoclastogenesis induction. B The protein expression level of angiogenesis and osteoclastogenesis makers in RA-FLSs from WT and miR-378high mice upon thapsigargin activation. C Images of tube formation at 3 and 6 h post-induction with the treatment of conditioned medium from miR-378high FLSs upon thapsigargin stimulation. D, E Semiquantitative analysis of total branching length of each group (D) and number of junctions (E) in each group (n = 6; *p < 0.05, **p < 0.01). F TRAP staining assay at day 5 with the treatment of conditioned medium from miR-378high FLSs upon thapsigargin stimulation. Scale bar: 100 μm. G Quantitative data of osteoclast fold change (n = 6; *p < 0.05, **p < 0.01). H The mRNA expression level of osteoclastogenesis-related markers measured by qRT-PCR (n = 6; *p < 0.05, **p < 0.01).

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