Fig. 6: Inhibition of ubiquitination of ME2 by PRMT1 mediates the upregulation of its protein stability. | Cell Death & Disease

Fig. 6: Inhibition of ubiquitination of ME2 by PRMT1 mediates the upregulation of its protein stability.

From: PRMT1-mediated methylation of ME2 promotes hepatocellular carcinoma growth by inhibiting ubiquitination

Fig. 6

A The effect of PRMT1 on the upregulation of ME2 expression was analyzed after the addition of chloroquine (lysosomal inhibitor) and MG132 (proteasome inhibitor) to cells. B ME2 expression was detected by immunoblotting after transfection with different doses of PRMT1. C ME2 expression was detected by immunoblotting after transfection with PRMT1 for different times. D Protein stability analysis of ME2 expression at different time points after addition of CHX to cells. E Ubiquitination assay to detect the ubiquitination level of ME2 after overexpression of PRMT1. F Ubiquitination assay to detect the ubiquitination level of ME2 in cells after disruption of PRMT1. G Ubiquitination assay to detect the ubiquitination level of ME2 in cells after mutation of PRMT1. H The changes of pS9-ME2 and meR67K-ME2 by IP, and the interaction between ME2 and AKT were detected both in HepG2 cells and Hep3B cells when wild and mutant HA-ME2 (WT, R67K) were transiently overexpressed. I, J. The amount of pS9-ME2 and the ME2-AKT interaction were detected by IP HA-ME2 after over-expressing PRMT1 (I) or silencing PRMT1 (J) in HepG2 cells and Hep3B cells, respectively.

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