Fig. 7: PRMT1-mediated methylation of ME2 inhibits ME2-FBW7 interactions and ubiquitination. | Cell Death & Disease

Fig. 7: PRMT1-mediated methylation of ME2 inhibits ME2-FBW7 interactions and ubiquitination.

From: PRMT1-mediated methylation of ME2 promotes hepatocellular carcinoma growth by inhibiting ubiquitination

Fig. 7

A Conserved methylation sites of ME2 were analyzed in multiple species. B, C Analysis of the expression levels of ME2 in wild type and mutants. D Analysis of changes in protein stability of ME2 wild-type and mutants. E Immunoprecipitation assay to analyze the interaction of ME2 with its E3 ubiquitination ligase FBW7. F, G Exogenous immunoprecipitation assay to analyze the interaction of FBW7 with ME2. H Exogenous immunoprecipitation assay to analyze PRMT1 inhibition of FBW7 interactions with ME2. I Endogenous immunoprecipitation analysis of PRMT1 inhibits the interaction between FBW7 and ME2. J Methylation of ME2 inhibits the interaction between FBW7 and ME2. K FBW7 promotes ubiquitination of ME2 as analyzed by ubiquitination assay. L Ubiquitination assay analysis showing that FBW7 can promote the ubiquitination level of ME2-K48. M Ubiquitination assay analysis showing that FBW7 modifies ubiquitination of wild-type and methylated ME2.

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