Fig. 5: GMFB modulates actin-filament organization in BMSCs. | Cell Death & Disease

Fig. 5: GMFB modulates actin-filament organization in BMSCs.

From: Glial maturation factor-β deficiency prevents oestrogen deficiency-induced bone loss by remodelling the actin network to suppress adipogenesis of bone marrow mesenchymal stem cells

Fig. 5

A, B Representative immunofluorescent images (A) in 48 h after seeding BMSCs with F-actin stained (phalloidin, red). Scale bars: 50 μm. Quantification of actin means fluorescent intensity (MFI) (B) of 48 h after seeding BMSCs. C Heatmap showing the gene expression values of indicated genes (RNA-seq) associated with actin-filament organization in comparison with GMFB KO and WT groups (n = 3). D–F Schematic for treatment 48 h after seeding of BMSCs with adipocyte differentiation medium and latrunculin B for actin-filament polymerization assay (D). Representative immunofluorescent images (E) in the cells with F-actin stained (phalloidin, red). Scale bar: 50 μm. Quantification of actin MFI (F) of BMSCs following treatment with different phases.

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